5USF
Leishmania donovani tyrosyl-tRNA synthetase in complex with nanobody and inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.180, 96.180, 351.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.290 - 2.750 |
| R-factor | 0.19144 |
| Rwork | 0.189 |
| R-free | 0.24353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p0j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.438 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.290 |
| High resolution limit [Å] | 2.750 |
| Rmerge | 0.210 |
| Number of reflections | 47547 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 100.0 |
| Redundancy | 10.3 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 298 | 0.1 M sodium cacodylate pH 5.7, 22% PEG 4,000, vapor diffusion, sitting drop, temperature 298 |
