5URZ
Crystal structure of Frizzled 5 CRD in complex with BOG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-09-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.99999 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 123.051, 123.051, 46.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.556 - 2.202 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.22360 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.215 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 20901 |
<I/σ(I)> | 25.4 |
Completeness [%] | 99.9 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M sodium citrate tribasic dehydrate pH 5.5, 22% polyethylene glycol 3350 |