5UQL
Clostridium difficile toxin A (TcdA) glucosyltransferase domain in complex with U2F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 |
| Unit cell lengths | 142.116, 142.116, 63.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.518 - 1.970 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3srz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.518 |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 51462 |
| <I/σ(I)> | 21.35 |
| Completeness [%] | 99.9 |
| Redundancy | 8.2 |
| CC(1/2) | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1 M HEPES 0.2 M L-proline PEG 3350, 10-25% |
