5UQE

Multidomain structure of human kidney-type glutaminase(KGA/GLS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 42 21 2
Unit cell lengths	144.420, 144.420, 615.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.642 - 3.600
R-factor	0.2762
Rwork	0.274
R-free	0.31270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.566
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (dev_2733)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.642	49.640	3.670
High resolution limit [Å]	3.600	18.000	3.600
Rmerge	0.349	0.067	2.214
Rmeas	0.357	0.068	2.269
Rpim	0.074	0.015	0.494
Number of reflections	143726
<I/σ(I)>	8.8
Completeness [%]	100.0	95.7	100
Redundancy	23.4	19.8	20.8
CC(1/2)	0.998	0.995	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	BIS-TRIS propane 0.1 M pH 6,8; Sodium Formate 1.8 M; Sodium Chloride 0.5 M; BPTES 1.2 mM