5UPL

CDC42 binds PAK4 via an extended GTPase-effector inteface - 2 peptide: PAK4FL, CDC42 - UNREFINED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	P 63
Unit cell lengths	141.652, 141.652, 62.093
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.637 - 3.003
R-factor	0.2593
Rwork	0.257
R-free	0.31020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fie
RMSD bond length	0.023
RMSD bond angle	1.617
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.637	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.117
Rpim	0.034	0.707
Number of reflections	14433
<I/σ(I)>	20.3	1.6
Completeness [%]	99.9	100
Redundancy	18
CC(1/2)	0.588	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M Tris-HCl pH 8.5, 50mM Na2SO4, 6% PEG6000