5UMU

Crystal structure of the middle double PH domain of human FACT complex subunit SPT16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	50.221, 99.819, 120.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 1.903
R-factor	0.1581
Rwork	0.157
R-free	0.19110
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.688
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.096	0.613
Number of reflections	91465	4677
<I/σ(I)>	23.8
Completeness [%]	99.8	100
Redundancy	10.4	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	2% Tacsimate pH 5.0, 100 mM Sodium citrate tribasic dihydrate pH 5.6, 10-20% Polyethylene glycol 3350