5UM9
Flap endonuclease 1 (FEN1) D86N with 5'-flap substrate DNA and Sm3+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.846, 105.846, 100.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.672 - 2.805 |
R-factor | 0.2107 |
Rwork | 0.208 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.568 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.672 |
High resolution limit [Å] | 2.805 |
Number of reflections | 30759 |
<I/σ(I)> | 31.28 |
Completeness [%] | 99.0 |
Redundancy | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | 6% MPEG 2K, 10% KCl, 50 mM HEPES pH 7.5, 2.5% Ethylene glycol, 1.06 mM SmSO4 |