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5UM9

Flap endonuclease 1 (FEN1) D86N with 5'-flap substrate DNA and Sm3+

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-2
Synchrotron siteSSRL
BeamlineBL12-2
Temperature [K]100
Detector technologyPIXEL
Collection date2016-07-14
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.97946
Spacegroup nameP 31 2 1
Unit cell lengths105.846, 105.846, 100.709
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.672 - 2.805
R-factor0.2107
Rwork0.208
R-free0.26590
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle0.568
Data reduction softwareHKL-2000
Data scaling softwareSCALA
Phasing softwarePHASER (2.5.6)
Refinement softwarePHENIX
Data quality characteristics
 Overall
Low resolution limit [Å]19.672
High resolution limit [Å]2.805
Number of reflections30759
<I/σ(I)>31.28
Completeness [%]99.0
Redundancy16.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2836% MPEG 2K, 10% KCl, 50 mM HEPES pH 7.5, 2.5% Ethylene glycol, 1.06 mM SmSO4

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