5UM9

Flap endonuclease 1 (FEN1) D86N with 5'-flap substrate DNA and Sm3+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 31 2 1
Unit cell lengths	105.846, 105.846, 100.709
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.672 - 2.805
R-factor	0.2107
Rwork	0.208
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.568
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	19.672
High resolution limit [Å]	2.805
Number of reflections	30759
<I/σ(I)>	31.28
Completeness [%]	99.0
Redundancy	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	6% MPEG 2K, 10% KCl, 50 mM HEPES pH 7.5, 2.5% Ethylene glycol, 1.06 mM SmSO4