5UM8

Crystal structure of HIV-1 envelope trimer 16055 NFL TD CC (T569G) in complex with Fabs 35022 and PGT124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 63
Unit cell lengths	126.456, 126.456, 314.093
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.247 - 3.935
R-factor	0.2634
Rwork	0.260
R-free	0.32850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez 4r26
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	4.020
High resolution limit [Å]	3.935	3.935
Rmerge	0.194	1.510
Rmeas	0.217	1.690
Rpim	0.096	0.750
Number of reflections	25153	1224
<I/σ(I)>	7.5	1.1
Completeness [%]	100.0	100
Redundancy	5	4.9
CC(1/2)	0.923	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.03	277	0.2M ammonium sulfate, 10% glycerol, 22% PEG 300, 0.1M phosphate-citrate