5ULK

Crystal Structure of RNF165 in complex with a UbcH5b~Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	69.109, 73.714, 121.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.780 - 2.380
R-factor	0.2096
Rwork	0.206
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.484
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.779	31.515	2.510
High resolution limit [Å]	2.381	7.530	2.380
Rmerge		0.042	0.734
Rmeas	0.074	0.046	0.807
Rpim	0.028	0.018	0.325
Total number of observations	85553	2531	8615
Number of reflections	12481
<I/σ(I)>	13.3	34	1.9
Completeness [%]	97.5	91.5	85.2
Redundancy	6.9	6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200 mM ammonium nitrate, 20% PEG 3350