5ULF
Crystal Structure of a UbcH5b~Ub conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.336, 61.356, 62.824 |
| Unit cell angles | 102.84, 91.66, 90.02 |
Refinement procedure
| Resolution | 48.518 - 1.800 |
| R-factor | 0.2291 |
| Rwork | 0.227 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.435 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.520 | 48.520 | 1.840 |
| High resolution limit [Å] | 1.800 | 8.990 | 1.800 |
| Rmerge | 0.103 | 0.034 | 0.928 |
| Rmeas | 0.119 | 0.040 | 1.089 |
| Rpim | 0.060 | 0.021 | 0.566 |
| Total number of observations | 150520 | 1142 | 6832 |
| Number of reflections | 38416 | ||
| <I/σ(I)> | 10 | 34.8 | 1.5 |
| Completeness [%] | 96.8 | 98.1 | 80 |
| Redundancy | 3.9 | 3.6 | 3.6 |
| CC(1/2) | 0.997 | 0.997 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 100 mM bis-Tris pH 5.5, 150 mM ammonium sulfate, 20% PEG 3350 |
