5UJ7
Structure of the active form of human Origin Recognition Complex ATPase motor module, complex subunitS 1, 4, 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 120.887, 81.141, 151.947 |
Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
Resolution | 19.882 - 3.394 |
R-factor | 0.245 |
Rwork | 0.242 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xgc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.520 |
High resolution limit [Å] | 3.390 | 3.390 |
Number of reflections | 39700 | |
<I/σ(I)> | 8.75 | 1.2 |
Completeness [%] | 97.0 | 96 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 22% Ethylene glycol 8% Propylene glycol 10mg/ml protein 1:1 ratio |