5UHM

Apo-Structure of Mature Growth Differentiation Factor 11

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.10537
Spacegroup name	P 32 2 1
Unit cell lengths	65.160, 65.160, 101.942
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.371 - 1.900
R-factor	0.2186
Rwork	0.217
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4g
RMSD bond length	0.007
RMSD bond angle	0.853
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.970	50.970	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.069	0.041	0.706
Rmeas	0.077	0.047	0.775
Rpim	0.032	0.021	0.316
Number of reflections	20340
<I/σ(I)>	10.4
Completeness [%]	100.0	99.6	100
Redundancy	5.8	5.2	5.9
CC(1/2)	0.996	0.996	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.75	298.15	5mM Calcium Chloride 0.1M Sodium Acetate 33% MPD