5UDL

IFIT1 N216A monomeric mutant (L457E/L464E) with m7Gppp-AAAA (anti conformation of cap)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	94
Detector technology	CCD
Collection date	2016-02-05
Detector	RAYONIX MX300HE
Wavelength(s)	0.979
Spacegroup name	P 42 2 2
Unit cell lengths	111.760, 111.760, 92.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.074 - 1.650
R-factor	0.1652
Rwork	0.164
R-free	0.18190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.005
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2142)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.096	0.049
Rmeas	0.099
Rpim	0.028
Total number of observations	1048686
Number of reflections	71832
<I/σ(I)>	8
Completeness [%]	100.0	99.8	99.9
Redundancy	14.6	13.9	12.3
CC(1/2)		0.999	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	277.15	27 - 32 % PEG 200, 0.1 M Tris pH 8.1, 200 mM CaCl2