5UDJ

IFIT1 monomeric mutant (L457E/L464E) with Gppp-AAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	94
Detector technology	CCD
Collection date	2016-01-16
Detector	RAYONIX MX300HE
Wavelength(s)	0.979
Spacegroup name	P 42 2 2
Unit cell lengths	112.536, 112.536, 93.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.299 - 1.690
R-factor	0.1602
Rwork	0.159
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.283
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2142)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.690	4.590	1.690
Rmerge	0.122	0.061
Number of reflections	67695
<I/σ(I)>	22.2		1.9
Completeness [%]	100.0	99.8	100
Redundancy	14.5	13.8	12.2
CC(1/2)		0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	277.15	27 - 32 % PEG 200, 0.1 M Tris pH 8.1, 200 mM CaCl2