5UD5

Crystal structure of the tRNA binding domain of Pyrrolysyl-tRNA synthetase bound to tRNA(Pyl)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979200
Spacegroup name	P 31 2 1
Unit cell lengths	72.280, 72.280, 238.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.218 - 2.347
R-factor	0.2191
Rwork	0.216
R-free	0.24230
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.220
High resolution limit [Å]	2.347
Number of reflections	31207
<I/σ(I)>	22.57
Completeness [%]	99.7
Redundancy	9.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	HEPES, magnesium chloride, PEG3350