5UBX
Crystal structure of a mutant mIgG2b Fc heterodimer in complex with Protein A peptide analog Z34C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.490, 101.490, 135.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.139 - 2.700 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rgs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.858 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (dev_1428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.053 | 0.802 |
| Number of reflections | 19956 | |
| <I/σ(I)> | 21.85 | 2.48 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.33 | 6.37 |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 17% PEG 3350, 0.2 M LiCl, 0.1 M Tris, pH 8.0 |
