5UBX
Crystal structure of a mutant mIgG2b Fc heterodimer in complex with Protein A peptide analog Z34C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Ig gamma-2B chain C region | polymer | 230 | 25880.5 | 1 | UniProt (P01867) Pfam (PF07654) In PDB | Mus musculus (Mouse) | |
| 2 | B | Ig gamma-2B chain C region | polymer | 236 | 26630.2 | 1 | UniProt (P01867) Pfam (PF07654) In PDB | Mus musculus (Mouse) | |
| 3 | Z | Peptide analog of B-domain from Protein A - Z34C | polymer | 34 | 4190.7 | 1 | Staphylococcus aureus | ||
| 4 | C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1114.0 | 1 | In PDB GlyTouCan (G55061AW) | |||
| 5 | D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1463.3 | 1 | In PDB GlyTouCan (G80858MF) | |||
| 6 | water | water | 18.0 | 42 | Chemie (HOH) |
Sequence modifications
A: 220 - 447 (UniProt: P01867)
B: 220 - 447 (UniProt: P01867)
| PDB | External Database | Details |
|---|---|---|
| Gly 218 | - | expression tag |
| Ser 219 | - | expression tag |
| Thr 307 | Pro 195 | engineered mutation |
| Leu 309 | Gln 197 | engineered mutation |
| Lys 370 | Val 258 | engineered mutation |
| Arg 409 | Lys 297 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 212 | - | expression tag |
| His 213 | - | expression tag |
| His 214 | - | expression tag |
| His 215 | - | expression tag |
| His 216 | - | expression tag |
| His 217 | - | expression tag |
| Gly 218 | - | expression tag |
| Ser 219 | - | expression tag |
| Asp 253 | Ile 141 | engineered mutation |
| Leu 405 | Phe 293 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 56701.3 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 2577.4 | |
| All* | Total formula weight | 59278.7 |
