5UB5

human POGLUT1 in complex with human Notch1 EGF12 S458T mutant and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-01
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	70.951, 74.072, 83.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.896 - 2.089
R-factor	0.1547
Rwork	0.153
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l0r
RMSD bond length	0.012
RMSD bond angle	1.235
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.900	2.164
High resolution limit [Å]	2.089	2.089
Rmerge	0.091	0.549
Number of reflections	26486	2401
<I/σ(I)>	18.74	3.16
Completeness [%]	99.0	91.49
Redundancy	11	5.3
CC(1/2)	0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% (v/v) PEG5000 MME, 50 mM MES pH 6.5, 2mM CaCl2, 250mM NaCl, 5% (v/v) 2-methyl-2,4-pentanediol