5UAJ
Escherichia coli RNA polymerase RpoB S531L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97690 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 187.219, 204.426, 310.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.959 - 3.915 |
| R-factor | 0.2607 |
| Rwork | 0.260 |
| R-free | 0.30730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yg2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.950 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 86257 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 81.3 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT |
