5U8W

Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa bound to NADH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	65.510, 117.830, 139.730
Unit cell angles	90.00, 94.30, 90.00

Refinement procedure

Resolution	34.935 - 1.790
R-factor	0.1668
Rwork	0.167
R-free	0.17850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lpf
RMSD bond length	0.004
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.280	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.085	1.410
Number of reflections	198646
<I/σ(I)>	17.3	2.7
Completeness [%]	100.0	100
Redundancy	13.5	13.8
CC(1/2)	0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	PEG 3350, HEPES, KCl, microseeding