5U81
Acid ceramidase (ASAH1, aCDase) from naked mole rat, Cys143Ala, uncleaved
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.767, 53.913, 48.802 |
| Unit cell angles | 90.00, 104.41, 90.00 |
Refinement procedure
| Resolution | 37.391 - 1.400 |
| R-factor | 0.124 |
| Rwork | 0.122 |
| R-free | 0.15840 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.391 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 69316 |
| <I/σ(I)> | 27.8 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | MES glycerol PEG 1000 PEG 600 |
