5U68

Structural basis for antibody cross-neutralization of respiratory syncytial virus and human metapneumovirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-13
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 31 2 1
Unit cell lengths	155.540, 155.540, 275.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.191 - 3.083
R-factor	0.187
Rwork	0.184
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jhw 3h42
RMSD bond length	0.021
RMSD bond angle	1.893
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.191
High resolution limit [Å]	3.100
Number of reflections	71891
<I/σ(I)>	11.96
Completeness [%]	100.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1M Potassium Nitrate, 0.1M Citrate Phosphate pH 4.2, 1 % Tacsimate pH 7.0, 14 (w/v) % PEG 6000