5U5Q

12 Subunit RNA Polymerase II at Room Temperature collected using SFX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2015-05-26
Detector	CS-PAD CXI-2
Wavelength(s)	1.55
Spacegroup name	C 2 2 21
Unit cell lengths	223.400, 396.800, 287.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.611 - 3.800
R-factor	0.219
Rwork	0.218
R-free	0.27060
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Refinement software	PHENIX ((dev_2356: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.611
High resolution limit [Å]	3.800
Number of reflections	123899
<I/σ(I)>	2.41
Completeness [%]	99.0
Redundancy	54.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.3	289.65	21-22% PEG 400, 5% PEG 5%, 100 mM Ammonium Phosphate pH 6.3, 100 mM NaCl, 1 mM ZnCl2 and 10 mM DTT