5U5P

Crystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus Musculus Complexed with a MLH1 NLS Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-01
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	77.215, 89.071, 99.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.579 - 2.171
R-factor	0.183
Rwork	0.181
R-free	0.21080
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1q1s
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.170
Number of reflections	36607
<I/σ(I)>	24.11
Completeness [%]	99.5
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.600-0.625 M sodium citrate (pH 6) 10 mM DTT