5U4R
Crystal structure of the broadly neutralizing Influenza A antibody VRC 315 53-1A09 Fab.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.553, 84.131, 79.273 |
| Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
| Resolution | 33.717 - 1.762 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.717 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 92183 |
| <I/σ(I)> | 14.39 |
| Completeness [%] | 98.7 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M sodium acetate, pH 4.6, 0.24 M ammonium acetate 26% (w/v) PEG-4000 |
