5U4P

Protein-protein complex between 26S proteasome regulatory subunit RPN8, RPN11, and Ubiquitin S31

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 65 2 2
Unit cell lengths	126.210, 126.210, 139.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	109.301 - 2.500
R-factor	0.2125
Rwork	0.211
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o8x 2znv 4boz 4jqw 4k1r rMSM 4un2
RMSD bond length	0.015
RMSD bond angle	1.198
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	109.301
High resolution limit [Å]	2.500
Rmerge	0.087
Number of reflections	23105
<I/σ(I)>	30.3
Completeness [%]	99.0
Redundancy	19.2
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	291	1.5 M ammonium tartrate