5U34

Crystal structure of AacC2c1-sgRNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	83.471, 128.198, 155.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.330 - 3.255
R-factor	0.2719
Rwork	0.270
R-free	0.32350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u31
RMSD bond length	0.001
RMSD bond angle	0.401
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.480
High resolution limit [Å]	3.250	3.250
Number of reflections	26140
<I/σ(I)>	10.2	1
Completeness [%]	99.2	95.6
Redundancy	5.9	5.7
CC(1/2)	0.990	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.2	293	0.2 M lithium sulfate, 0.1 M HEPES (pH 7.5), and 25% PEG3350 (v/v)