5U34

Crystal structure of AacC2c1-sgRNA binary complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	Region: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Region: {"description":"Binds non-target ssDNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	1
Details	Site: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Site: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Binds 'phosphate lock' on target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Site: {"description":"Binds 'phosphate lock' on target strand DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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