5U34
Crystal structure of AacC2c1-sgRNA binary complex
Functional Information from GO Data
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 3 |
Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000305|PubMed:27984729, ECO:0000305|PubMed:27989439 |
Chain | Residue | Details |
A | ASP570 | |
A | ALA848 | |
A | ASP977 |
site_id | SWS_FT_FI2 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000269|PubMed:27984729, ECO:0000269|PubMed:27989439 |
Chain | Residue | Details |
A | SER899 | |
A | ARG911 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | SITE: Binds DNA protospacer adjacent motif (PAM) on target DNA => ECO:0000269|PubMed:27984729 |
Chain | Residue | Details |
A | ASN400 |
site_id | SWS_FT_FI4 |
Number of Residues | 10 |
Details | SITE: Binds sgRNA => ECO:0000269|PubMed:27984729, ECO:0000269|PubMed:27989439 |
Chain | Residue | Details |
A | ARG415 | |
A | GLN982 | |
A | ARG484 | |
A | TYR501 | |
A | PHE600 | |
A | HIS614 | |
A | ARG734 | |
A | GLN767 | |
A | TYR825 | |
A | GLN882 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | SITE: Binds 'phosphate lock' on target strand DNA; via amide nitrogen => ECO:0000269|PubMed:27984729 |
Chain | Residue | Details |
A | GLY478 |
site_id | SWS_FT_FI6 |
Number of Residues | 1 |
Details | SITE: Binds 'phosphate lock' on target strand DNA => ECO:0000269|PubMed:27984729 |
Chain | Residue | Details |
A | ARG507 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | SITE: Disrupts base stacking adjacent to scissile phosphate => ECO:0000305|PubMed:27984729 |
Chain | Residue | Details |
A | TYR853 |