Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.652, 80.381, 81.178 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.600 |
| R-factor | 0.1832 |
| Rwork | 0.180 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v2y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.010 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.194 | 0.070 | 1.411 |
| Number of reflections | 19781 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 96.9 | 94.2 | 96.8 |
| Redundancy | 5.4 | 5.3 | 5.1 |
| CC(1/2) | 0.995 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.5 | 293 | 31% PEG400, 100mM Sodium Citrate pH5.5, 100mM magnesium sulfate |
