5TZ2
Crystal structure of human CD47 ECD bound to Fab of C47B222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.567, 72.934, 72.698 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 32.216 - 2.302 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jjs |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.216 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 | 0.432 |
| Number of reflections | 26271 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.8 | 97 |
| Redundancy | 3.1 | 2.8 |
| CC(1/2) | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 25% PEG 3000, 1M LiCl, 0.1M MES pH 6.5 |
