5TYU

DNA Polymerase Mu Reactant Complex, Mn2+ (4 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-17
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.155, 62.223, 118.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.366 - 2.048
R-factor	0.1881
Rwork	0.187
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.094	0.043	0.857
Rmeas	0.104
Rpim	0.044
Total number of observations	157686
Number of reflections	28596
<I/σ(I)>	7.8
Completeness [%]	99.0	99.6	98.3
Redundancy	5.5	5.4	5.5
CC(1/2)		0.998	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000