5TXK

CRYSTAL STRUCTURE OF USP35 C450S IN COMPLEX WITH UBIQUITIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-03
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	104.235, 104.235, 106.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 1.840
R-factor	0.1611
Rwork	0.160
R-free	0.18340
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	BUSTER-TNT (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.380
High resolution limit [Å]	1.840
Number of reflections	51309
<I/σ(I)>	37
Completeness [%]	100.0
Redundancy	37.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	0.95 M ammonium sulphate 0.1 M HEPES