5TWL

Structure of Maternal Embryonic Leucine Zipper Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	57.520, 68.300, 104.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.767 - 2.420
R-factor	0.1983
Rwork	0.196
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	38.770
High resolution limit [Å]	2.420
Number of reflections	15943
<I/σ(I)>	12.52
Completeness [%]	97.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	magnesium chloride, PEG 3350, BIS-TRIS