5TV1
active arrestin-3 with inositol hexakisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-04-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 63 |
Unit cell lengths | 97.575, 97.575, 76.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.030 - 2.400 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g4m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.285 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.030 |
High resolution limit [Å] | 2.400 |
Number of reflections | 15997 |
<I/σ(I)> | 26.2 |
Completeness [%] | 97.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 mM Succinate/Phosphate/Glycine pH 8.5 and 25% PEG 1500 |