5TUD
Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.56 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.290, 118.860, 145.380 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 35.640 - 3.000 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nc3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.760 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.640 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 48606 | |
| <I/σ(I)> | 4.3 | 0.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 310.3 | 120.26 |
| CC(1/2) | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.7 | 293 | Tris/HCL pH 7.7, 60 mM Sodium/Potassium Tartrate, 25% PEG400. |
