5TUD

Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	5-hydroxytryptamine receptor 2B,Soluble cytochrome b562 chimera	polymer	457	51497.0	2	UniProt (P41595) UniProt (P0ABE7) Pfam (PF00001) Pfam (PF07361)	Homo sapiens (Human)	5-HT2B,Serotonin receptor 2B,Cytochrome b-562
2	B, E (B, E)	Anti-5-HT2B Fab light chain	polymer	213	23151.8	2		Mus musculus (Mouse)
3	C, F (C, F)	Anti-5-HT2B Fab heavy chain	polymer	236	25483.5	2		Mus musculus (Mouse)
4	G, H (A, D)	Ergotamine	non-polymer		581.7	2	Chemie (ERM)

Sequence modifications

A, D: 36 - 248 (UniProt: P41595)

PDB	External Database	Details
Met 10	-	initiating methionine
Lys 11	-	expression tag
Thr 12	-	expression tag
Ile 13	-	expression tag
Ile 14	-	expression tag
Ala 15	-	expression tag
Leu 16	-	expression tag
Ser 17	-	expression tag
Tyr 18	-	expression tag
Ile 19	-	expression tag
Phe 20	-	expression tag
Cys 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Phe 24	-	expression tag
Ala 25	-	expression tag
Asp 26	-	expression tag
Tyr 27	-	expression tag
Lys 28	-	expression tag
Asp 29	-	expression tag
Asp 30	-	expression tag
Asp 31	-	expression tag
Asp 32	-	expression tag
Gly 33	-	expression tag
Ala 34	-	expression tag
Pro 35	-	expression tag
Trp 144	Met 144	engineered mutation

A, D: 1001 - 1106 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 1007	Met 29	engineered mutation
Ile 1102	His 124	engineered mutation
Leu 1106	Arg 128	engineered mutation

A, D: 314 - 405 (UniProt: P41595)

PDB	External Database	Details
Gly 406	-	expression tag
Arg 407	-	expression tag
Pro 408	-	expression tag
Leu 409	-	expression tag
Glu 410	-	expression tag
Val 411	-	expression tag
Leu 412	-	expression tag
Phe 413	-	expression tag
Gln 414	-	expression tag
Gly 415	-	expression tag
Pro 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	200264.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1163.3
All*	Total formula weight	201428.0

*Water molecules are not included.