5TSR
Crystal structure of PRL-3 phosphatase in complex with the Bateman domain of CNNM3 magnesium transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.339, 125.146, 52.042 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 27.738 - 3.188 |
| R-factor | 0.2413 |
| Rwork | 0.239 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k22 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.188 | 3.188 |
| Rmerge | 0.534 | |
| Number of reflections | 15609 | |
| <I/σ(I)> | 10.5 | 1.5 |
| Completeness [%] | 96.6 | 99.6 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.0, 0.064 M Tri-Na Citrate, 15% PEG 5000 MME |
