5TRM

Crystal structure of human GCN5 histone acetyltransferase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	77
Detector technology	CCD
Collection date	2015-08-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	173.474, 173.474, 347.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.179 - 2.900
R-factor	0.2181
Rwork	0.215
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z4r
RMSD bond length	0.009
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2152)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.118	0.047	0.543
Rmeas	0.129	0.051	0.600
Rpim	0.051	0.018	0.252
Total number of observations	726744
Number of reflections	117491
<I/σ(I)>	6.3
Completeness [%]	99.7	97.9	100
Redundancy	6.2	7.7	5.6
CC(1/2)		0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1.8M Sodium formate, 0.1M Sodium acetate pH 4.6