5TKM

Crystal structure of human APOBEC3B N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	60.739, 60.802, 111.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.370 - 1.900
R-factor	0.1886
Rwork	0.188
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k81
RMSD bond length	0.007
RMSD bond angle	0.793
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.066
Number of reflections	33189
<I/σ(I)>	29.19
Completeness [%]	99.8
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.01 M Iron (III) chloride hexahydrate, 0.1 M sodium citrate dehydrate pH 5.6, 10% v/v Jeffamine M-600