5TJG
Thermus aquaticus delta1.1-sigmaA holoenzyme/downstream-fork promoter complex with an open clamp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 104.098, 111.423, 143.177 |
| Unit cell angles | 104.12, 99.66, 109.43 |
Refinement procedure
| Resolution | 38.627 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cw0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.627 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 1.200 |
| Number of reflections | 169540 | |
| <I/σ(I)> | 16.5 | 0.9 |
| Completeness [%] | 97.1 | 91.3 |
| Redundancy | 3 | 2.8 |
| CC(1/2) | 0.913 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M MES, pH 6.5, 50 mM MgCl2, 5-8% (w/v) polyethylene glycol, 10% (v/v) isopropanol |
