5TIB
Gasdermin-B C-terminal domain containing the polymorphism residues Arg299:Ser306 fused to maltose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-04-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.060, 104.060, 252.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.190 - 2.600 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3H4Z |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 96.200 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.112 |
| Number of reflections | 41354 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.9 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 2.2 M sodium malonate, and 9 mM hexamine CoCl3 |
