5TGS
Crystal Structure of QueE from Bacillus subtilis with methionine bound
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9999 , 1.7389 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.182, 79.297, 122.224 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.011 - 2.548 |
R-factor | 0.2173 |
Rwork | 0.216 |
R-free | 0.24820 |
Structure solution method | MAD |
RMSD bond length | 0.076 |
RMSD bond angle | 2.128 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.548 | 2.548 |
Number of reflections | 17745 | |
<I/σ(I)> | 17.7 | 2.3 |
Completeness [%] | 99.3 | 98 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 MTris HCl (pH 8.5), 22% PEG 8000 and 0.1 M Magnesium chloride |