5TF6
Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.921, 84.446, 255.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.300 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.23160 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.771 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.100 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.090 |
Number of reflections | 141843 |
<I/σ(I)> | 17.67 |
Completeness [%] | 99.7 |
Redundancy | 12.1 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1 M lithium chloride, 100 mM sodium MES, 64 mM hydrochloric acid, 25% PEG 8,000, 20% glycerol, 1 mM MnCl2, 400 mM potassium chloride, 10 mM HEPES acid, 10 mM Tris base, 2 mM MgCl2, 1 mM TCEP-HCl |