5TDB
Crystal structure of the human UBR-box domain from UBR2 in complex with asymmetrically double methylated arginine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6362 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.573, 37.168, 29.746 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 15.463 - 1.101 |
| R-factor | 0.1252 |
| Rwork | 0.125 |
| R-free | 0.13400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.463 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 24588 | |
| <I/σ(I)> | 32.6 | 2.03 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris pH 5.5 and 25% PEG 3350 |
