5T8F
p110delta/p85alpha with taselisib (GDC-0032)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.096, 108.604, 142.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.370 - 2.910 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dxu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.370 | 3.160 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.044 | |
| Number of reflections | 31603 | |
| <I/σ(I)> | 20.4 | 5.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG |
