5T7L

Pt(II)-mediated copper-dependent interactions between ATOX1 and MNK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0597
Spacegroup name	P 21 21 21
Unit cell lengths	47.617, 54.659, 63.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.486 - 2.830
R-factor	0.2035
Rwork	0.200
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cjk
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XDS
Phasing software	Sir2014
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.490	2.931
High resolution limit [Å]	2.830	2.830
Rmerge	0.107
Number of reflections	4184
<I/σ(I)>	7.58	2.41
Completeness [%]	98.1	92.77
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG-6000 10 to 30 % w/v and sodium citrate 0.1M at pH 4.7
1	VAPOR DIFFUSION, HANGING DROP		298	PEG-6000 10 to 30 % w/v and sodium citrate 0.1M at pH 4.7