5T4X
CRYSTAL STRUCTURE OF PDE6D IN APO-STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979080 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.662, 56.534, 89.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.740 - 1.810 |
| R-factor | 0.18446 |
| Rwork | 0.183 |
| R-free | 0.21368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jhp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.649 | |
| Number of reflections | 13356 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1M TRIS PH 9.0, 0.1M NAOAC PH 5.1, 8% PEG8000, 15% 2-PROPANOL, VAPOR DIFFUSION, TEMPERATURE 291K |
