5T4U
Crystal structure of the bromodomain of human BRPF1 in complex with a quinolinone ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.315, 60.315, 63.389 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.230 - 1.500 |
| R-factor | 0.20755 |
| Rwork | 0.206 |
| R-free | 0.23079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.230 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.350 |
| Number of reflections | 206811 | |
| <I/σ(I)> | 17.8 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 7.7 |
| CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 20 % PEG3350, 0.2 M ammonium nitrate |
