5T4N

Crystal Structure of Human Protocadherin-15 EC3-5 D414A Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 65
Unit cell lengths	105.655, 105.655, 193.767
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.380 - 2.701
Rwork	0.202
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t4m
RMSD bond length	0.012
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1819)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	2.798
High resolution limit [Å]	2.701	2.701
Rmerge	0.184	3.498
Number of reflections	33423
<I/σ(I)>	9.18	0.6
Completeness [%]	99.0	99
Redundancy	7	7
CC(1/2)	0.996	0.171

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	0.1 M Sodium Cacodylate pH 5.8, 150 mM Calcium Chloride