5T3Z
3.5 Angstrom Crystal Structure of a Fully and Natively Glycosylated BG505 SOSIP.664 HIV-1 Env Trimer in Complex with the Broadly Neutralizing Antibodies IOMA and 10-1074
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 217.258, 217.258, 154.935 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.630 - 3.500 |
| R-factor | 0.267 |
| Rwork | 0.265 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t3x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.839 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.631 | 3.670 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.680 | 9.290 |
| Rpim | 0.090 | 1.210 |
| Number of reflections | 34373 | 4992 |
| <I/σ(I)> | 12.6 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 61.1 | 59.7 |
| CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM ammonium citrate tribasic pH 7.0 100 mM imidazole pH 7.0 20% PEG MME 2,000 |
